A postdoctoral position is available immediately in
mitochondrial signaling in type 2 diabetes (T2D) at the Buck
Institute for Research on Aging, Novato, California (San Francisco
This is a full time position with an initial one-year contract
that can be extended.
The project uses pancreatic -cell physiology assaying and
top-down analysis of bioenergetic parameters measured using
fluorescence microscopy and Seahorse respirometry to uncover causes
of beta cell failure in T2D. Therefore both strong wet-bench and
data analysis skills are required.
Altered cellular energetics and related cellular signaling
contribute to human disease in general and physiological aging and
chronic diseases in particular, including cancer,
neurodegeneration, ischemic heart disease and type 2 diabetes.
Mitochondrial metabolism also plays a central role in insulin
secretion in pancreatic beta cells. When glucose is elevated, the
increased metabolism results in a substantial mitochondrial
membrane potential hyperpolarization, as well as in increased rates
of ATP synthesis and turnover marked by faster cell respiration.
The project uses microscale bioenergetic assaying of human primary
-cell cultures, assessment of -cell physiology and top-down
analysis of cellular energy metabolism. The project investigates
the molecular pathways that make healthy - cell bioenergetics - and
insulin secretion - normally highly responsive to glucose, and
pathways that may fail in type 2 diabetes. Relevant papers from our
lab to this project are: PMIDs 26204464, 27404273, 27771512.
The successful candidate will receive training in the particular
experimental technologies used in this project, will design and
conduct experiments and analyze data. The postdoc is expected to
lead his/her own project, write up and present results.
- PhD in biological or biomedical sciences, bioengineering or
- Solid knowledge of cell and mitochondrial metabolism
- Cell culturing experience
- Wet bench experience
- Proven publication record
- Strong written and oral communication skills
- Ability to prioritize and manage own time
- Ability to work independently and also capable of interacting
successfully within a group.
DESIRED SKILLS AND KNOWLEDGE:
- Cell or mitochondrial respirometry
- Fluorescence microscopy
- Experience in -cell physiology or in diabetes
- Experience in metabolic modeling
- Experience in RNAi methods
BUCK INSTITUTE ENVIRONMENT:
The Buck Institute for Research on Aging consists of
state-of-the-art research laboratories with faculty recruited from
four different disciplines - diseases of aging, processes of aging,
stem cell biology, and technology for aging studies. The labs were
intentionally designed for close proximity interactions without
departmental boundaries. Since its inception, the Buck Institute
has collaborated closely with UCSF, UCB, LBNL and other San
Francisco Bay Area researchers, clinicians and patient advocates at
many levels. About 25% of Buck Institute faculty hold joint
academic appointments with UCSF and/or UCB-LBNL.
The Institute sits on 488 acres of Mt. Burdell in Marin County,
20 miles north of San Francisco. Long-term plans call for 550
employees in 45 faculty-supervised laboratories within five
buildings totaling 360,000 square feet; to date, the first three
buildings hold 200 employees in 18 laboratories and five cores. The
Institute is supported by funds from the Buck Trust (20%),
Government grants (NIH, CIRM, DOE; 35%), contributions and grants
from individuals, corporations and foundations (20%) and
corporate/philanthropic-sponsored agreements (25%). The Buck
Institute is a multi-disciplinary facility and houses research
associated with neurology, molecular genetics, molecular biology,
cell biology, neurochemistry, cancer biology, mass spectrometry,
proteomics, and apoptosis. Additional research support within the
Buck Institute campus includes a 238-seat auditorium. The Buck
Institute has developed several specialized multi-user core
equipment facilities including the Morphology and Imaging Core
(co-directed by Dr Akos Gerencser), a Bioinformatics and Genomics
Core equipped with high- throughput microarray robotics, plate
readers, laser capture microscope and multi-species genome probe
libraries, as well as one of the Bay Area's best equipped
Chemical/Proteomics/Mass Spectrometry Core facilities.
FOR ADDITIONAL INFORMATION:
Akos Gerencser MD. Ph.D.
Assistant Director of the Morphology and Imaging Core
Assistant Research Professor
T: (415) 209-2273